1DM4
SER195ALA MUTANT OF HUMAN THROMBIN COMPLEXED WITH FIBRINOPEPTIDE A (7-16)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 123 |
Detector technology | IMAGE PLATE |
Collection date | 1996-03-07 |
Detector | RIGAKU RAXIS II |
Spacegroup name | I 21 3 |
Unit cell lengths | 135.100, 135.100, 135.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.000 - 2.500 |
R-factor | 0.169 |
RMSD bond length | 0.016 |
RMSD bond angle | 0.042 |
Phasing software | AMoRE |
Refinement software | PROFFT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.750 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.093 | 0.190 |
Total number of observations | 21833 * | |
Number of reflections | 9561 | |
<I/σ(I)> | 15 | |
Completeness [%] | 71.0 | 61 * |
Redundancy | 2.28 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 277 * | 1.4M SODIUM CITRATE, 1-2% ISOPROPANOL, 0.1M HEPES BUFFER, pH 7.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | reservoir | sodium citrate | 1.4 (M) | |
3 | 1 | reservoir | HEPES | 0.1 (M) |