1DLA

NOVEL NADPH-BINDING DOMAIN REVEALED BY THE CRYSTAL STRUCTURE OF ALDOSE REDUCTASE

Experimental procedure

Spacegroup name	P 1
Unit cell lengths	81.300, 85.900, 56.600
Unit cell angles	102.30, 103.30, 79.00

Refinement procedure

Resolution	? - 3.000
R-factor	0.219
Rwork	0.219
RMSD bond length	0.020
RMSD bond angle	3.700
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	82.000 *
High resolution limit [Å]	2.500 *
Rmerge	0.065 *
Total number of observations	128597 *
Number of reflections	40833 *
Completeness [%]	82.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.2 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	5 (mg/ml)
2	1	drop	PEG6000	2.5 (%(w/v))
3	1	drop	citrate	10 (mM)
4	1	drop	dithiothreitol	1 (mM)
5	1	drop	sodium azide	1 (mM)
6	1	reservoir	PEG6000	20 (%(w/v))