1DKT
CKSHS1: HUMAN CYCLIN DEPENDENT KINASE SUBUNIT, TYPE 1 COMPLEX WITH METAVANADATE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 295 |
Detector technology | AREA DETECTOR |
Detector | SIEMENS-NICOLET X100 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 94.000, 94.000, 131.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 6.000 - 2.900 |
R-factor | 0.195 |
Rwork | 0.195 |
R-free | 0.25800 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.900 |
Data reduction software | XDS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.038 | 0.150 |
Number of reflections | 7655 | |
<I/σ(I)> | 25 | 5 |
Completeness [%] | 94.0 | 80 |
Redundancy | 3. * | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 25 (%(w/v)) | |
2 | 1 | drop | protein | 15-30 (mg/ml) | |
3 | 1 | drop | sodium metavanadate | 0.0012ml | |
4 | 1 | drop | reservoir solution | 0.008ml |