1DKT
CKSHS1: HUMAN CYCLIN DEPENDENT KINASE SUBUNIT, TYPE 1 COMPLEX WITH METAVANADATE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS-NICOLET X100 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 94.000, 94.000, 131.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 2.900 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.25800 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.900 |
| Data reduction software | XDS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.038 | 0.150 |
| Number of reflections | 7655 | |
| <I/σ(I)> | 25 | 5 |
| Completeness [%] | 94.0 | 80 |
| Redundancy | 3. * | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 25 (%(w/v)) | |
| 2 | 1 | drop | protein | 15-30 (mg/ml) | |
| 3 | 1 | drop | sodium metavanadate | 0.0012ml | |
| 4 | 1 | drop | reservoir solution | 0.008ml |
