1DKT

CKSHS1: HUMAN CYCLIN DEPENDENT KINASE SUBUNIT, TYPE 1 COMPLEX WITH METAVANADATE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	295
Detector technology	AREA DETECTOR
Detector	SIEMENS-NICOLET X100
Spacegroup name	P 61 2 2
Unit cell lengths	94.000, 94.000, 131.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.900
R-factor	0.195
Rwork	0.195
R-free	0.25800
RMSD bond length	0.009
RMSD bond angle	1.900
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.038	0.150
Number of reflections	7655
<I/σ(I)>	25	5
Completeness [%]	94.0	80
Redundancy	3. *	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	25 (%(w/v))
2	1	drop	protein	15-30 (mg/ml)
3	1	drop	sodium metavanadate		0.0012ml
4	1	drop	reservoir solution		0.008ml