1DJ9
CRYSTAL STRUCTURE OF 8-AMINO-7-OXONANOATE SYNTHASE (OR 7-KETO-8AMINIPELARGONATE OR KAPA SYNTHASE) COMPLEXED WITH PLP AND THE PRODUCT 8(S)-AMINO-7-OXONANONOATE (OR KAPA). THE ENZYME OF BIOTIN BIOSYNTHETIC PATHWAY.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 58.890, 58.890, 201.770 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.27800 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.020 |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.620 | 2.060 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.065 | 0.452 |
| Number of reflections | 27822 | |
| <I/σ(I)> | 6.3 | |
| Completeness [%] | 99.3 | 93.5 |
| Redundancy | 2.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 289 | AMMONIUM SULPHATE, SODIUM CHLORIDE, BIS-TRIS PROPANE BUFFER, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | bis-Tris/propane | 10 (mM) | |
| 3 | 1 | drop | 50-100 (mM) | ||
| 4 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
| 5 | 1 | reservoir | bis-Tris/propane | 200 (mM) | |
| 6 | 1 | reservoir | glycerol | 30 (%(v/v)) | |
| 7 | 1 | reservoir | AON | 0.1 (mM) |
