1DJ7
CRYSTAL STRUCTURE OF FERREDOXIN THIOREDOXIN REDUCTASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-07-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 45.296, 45.296, 172.147 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.600 |
| R-factor | 0.264 |
| Rwork | 0.238 |
| R-free | 0.27800 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.800 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.049 | 0.196 |
| Number of reflections | 26709 | |
| <I/σ(I)> | 10.93 | |
| Completeness [%] | 88.8 | |
| Redundancy | 3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 * | 297 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 17 (mg/ml) | |
| 2 | 1 | drop | triethanolamine-HCl | 20 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 4 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 5 | 1 | reservoir | 0.1 (M) | ||
| 6 | 1 | reservoir | 100 (mM) |
