1DHK

STRUCTURE OF PORCINE PANCREATIC ALPHA-AMYLASE

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-10-15
Detector	MAR scanner 180 mm plate
Spacegroup name	C 1 2 1
Unit cell lengths	151.600, 79.400, 68.000
Unit cell angles	90.00, 91.54, 90.00

Refinement procedure

Resolution	8.000 - 1.850
R-factor	0.183
Rwork	0.183
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PPA NATIVE AND LECTIN FROM SEEDS OF LATHYRUS OCHRUS
RMSD bond length	0.010
RMSD bond angle	1.480
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000 *	1.920
High resolution limit [Å]	1.850 *	1.850
Rmerge	0.050	0.406
Number of reflections	66769
<I/σ(I)>	14.8	2
Completeness [%]	96.7	98.4
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5 *		drop solution was mixed with an equal volume of reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	15 (mg/ml)
2	1	reservoir	sodium acetate	0.1 (M)
3	1	reservoir	MPD	20 (%)