1DH3
CRYSTAL STRUCTURE OF A CREB BZIP-CRE COMPLEX REVEALS THE BASIS FOR CREB FAIMLY SELECTIVE DIMERIZATION AND DNA BINDING
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 110.610, 49.320, 79.250 |
| Unit cell angles | 90.00, 122.18, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.000 |
| R-factor | 0.218 |
| Rwork | 0.223 |
| R-free | 0.28000 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.906 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | EPMR |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 3.200 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.065 * | 0.258 |
| Number of reflections | 7316 | |
| <I/σ(I)> | 17 | |
| Completeness [%] | 95.0 * | 87.4 * |
| Redundancy | 4.3 * | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.6 | 298 | PEG 8000, MGCL2, (NH4)2SO4, MES, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MGCL2 | ||
| 2 | 1 | 1 | (NH4)2SO4 | ||
| 3 | 1 | 1 | MES | ||
| 4 | 1 | 1 | PEG 8000 | ||
| 5 | 1 | 2 | PEG 8000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ZIP dimer | 1 (mM) | |
| 2 | 1 | drop | CRE duplex | 1 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 12 (%) | |
| 4 | 1 | reservoir | 10 (mM) | ||
| 5 | 1 | reservoir | ammonium sulfate | 100 (mM) | |
| 6 | 1 | reservoir | MES | 50 (mM) | pH5.6 |
