1DGE
AN ALKALI METAL ION SIZE-DEPENDENT SWITCH IN THE ACTIVE SITE STRUCTURE OF DIALKYLGLYCINE DECARBOXYLASE
Experimental procedure
Spacegroup name | P 64 2 2 |
Unit cell lengths | 153.500, 153.500, 86.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 2.800 |
R-factor | 0.195 |
RMSD bond length | 0.013 |
RMSD bond angle | 2.400 |
Refinement software | TNT |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.800 * |
Rmerge | 0.108 * |
Total number of observations | 62340 * |
Number of reflections | 14856 * |
Completeness [%] | 97.7 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | referred to J.Mol.Biol. 222.873-875 1991 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20-60 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 5 (mM) | |
3 | 1 | drop | PLP | 0.015 (mM) | |
4 | 1 | drop | sodium azide | 0.02 (%(w/v)) | |
5 | 1 | reservoir | PEG4000 | 15 (%(w/v)) | |
6 | 1 | reservoir | morpholinoethanesulfonic acid | 15 (mM) | |
7 | 1 | reservoir | potassium hydroxide | to neutralize | |
8 | 1 | reservoir | sodium pyruvate | 75-150 (mM) | |
9 | 1 | reservoir | PLP | 0.015 (mM) |