1DG9

CRYSTAL STRUCTURE OF BOVINE LOW MOLECULAR WEIGHT PTPASE COMPLEXED WITH HEPES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1996-01-01
Detector	RIGAKU RAXIS II
Spacegroup name	C 1 2 1
Unit cell lengths	95.300, 43.400, 41.200
Unit cell angles	90.00, 113.40, 90.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.179
RMSD bond length	0.010
RMSD bond angle	14.200 *
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.900	1.900
Rmerge	0.065
Total number of observations	71205 *
Number of reflections	12122 *
Completeness [%]	98.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5		20% PEG4k, 10% 2-propanol, 100mM HEPES, pH 7.5, VAPOR DIFFUSION

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	1	inorganic phosphate	2-5 (mM)
3	1	2	PEG4000	20 (%)
4	1	2	2-propanol	10 (%)
5	1	2	HEPES	100 (mM)