1DG1

WHOLE, UNMODIFIED, EF-TU(ELONGATION FACTOR TU).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1996-03-01
Detector	SDMS
Spacegroup name	P 1 21 1
Unit cell lengths	63.600, 104.600, 67.200
Unit cell angles	90.00, 97.02, 90.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.168
Rwork	0.168
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.282 *
Data reduction software	SDMS
Data scaling software	SDMS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.123
High resolution limit [Å]	2.500	2.500 *
Rmerge	0.068 *
Total number of observations	357998 *
Number of reflections	28782
Completeness [%]	94.9	91 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6 *	20 *	PEG 3350, ammonium citrate, ammonium acetate, pH 5.75, VAPOR DIFFUSION, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	50 (mM)
2	1	drop		10 (mM)
3	1	drop	2-mercaptoethanol	10 (mM)
4	1	drop	protein	21.1 (mg/ml)
5	1	reservoir	ammonium sulfate	0.2 (M)
6	1	reservoir	ammonium citrate	0.1 (M)
7	1	reservoir	PEG3350	24 (%(w/v))