1DG1
WHOLE, UNMODIFIED, EF-TU(ELONGATION FACTOR TU).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-03-01 |
| Detector | SDMS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.600, 104.600, 67.200 |
| Unit cell angles | 90.00, 97.02, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.168 |
| Rwork | 0.168 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.282 * |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.123 | |
| High resolution limit [Å] | 2.500 | 2.500 * |
| Rmerge | 0.068 * | |
| Total number of observations | 357998 * | |
| Number of reflections | 28782 | |
| Completeness [%] | 94.9 | 91 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.6 * | 20 * | PEG 3350, ammonium citrate, ammonium acetate, pH 5.75, VAPOR DIFFUSION, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 50 (mM) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | 2-mercaptoethanol | 10 (mM) | |
| 4 | 1 | drop | protein | 21.1 (mg/ml) | |
| 5 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 6 | 1 | reservoir | ammonium citrate | 0.1 (M) | |
| 7 | 1 | reservoir | PEG3350 | 24 (%(w/v)) |
