1DFU
CRYSTAL STRUCTURE OF E.COLI RIBOSOMAL PROTEIN L25 COMPLEXED WITH A 5S RRNA FRAGMENT AT 1.8 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-01-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.600, 76.600, 95.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.226 |
| Rwork | 0.207 |
| R-free | 0.22500 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.048 | 0.313 |
| Number of reflections | 24727 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 96.0 | 77 |
| Redundancy | 15 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | MPD, POTASSIUM CHLORIDE, MAGNESIUM CHLORIDE, CACODYLATE, pH 6.0, VAPOR DIFFUSION, SITTING DROP at 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | KCL | ||
| 2 | 1 | 1 | MGCL2 | ||
| 3 | 1 | 1 | CACODYLATE | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA-complex | 10 (mg/ml) | |
| 2 | 1 | drop | MPD | 20 (%) | |
| 3 | 1 | drop | Na cacodylate | 50 (mM) | |
| 4 | 1 | drop | 100 (mM) | ||
| 5 | 1 | drop | 10 (mM) | ||
| 6 | 1 | reservoir | MPD | 20 (%(v/v)) | |
| 7 | 1 | reservoir | ammonium sulfate | 0.4 (M) |
