1DEG
THE LINKER OF DES-GLU84 CALMODULIN IS BENT AS SEEN IN THE CRYSTAL STRUCTURE
Experimental procedure
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.300, 49.900, 62.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | ? - 2.900 |
R-factor | 0.23 |
Rwork | 0.230 |
RMSD bond length | 0.030 |
RMSD bond angle | 5.490 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 2.900 * | 2.900 * |
Rmerge | 0.090 * | 0.115 * |
Number of reflections | 3396 * | |
Redundancy | 3.8 * | 2.1 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.1 * | 4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 20 (%sat) | |
2 | 1 | reservoir | 10 (mM) | ||
3 | 1 | reservoir | MES | 50 (mM) | |
4 | 1 | drop | protein | 10 (mg/ml) |