1DE9
HUMAN APE1 ENDONUCLEASE WITH BOUND ABASIC DNA AND MN2+ ION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-05-21 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.060, 98.350, 101.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.1831 |
| Rwork | 0.183 |
| R-free | 0.27400 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.354 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.086 | 0.424 |
| Number of reflections | 17008 | |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 91.0 | 92.4 |
| Redundancy | 2.45 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 | 298 | MPEG 2000, LITHIUM SULFATE, MANGANESE CHLORIDE, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | LI2SO4 | ||
| 2 | 1 | 1 | MNCL2 | ||
| 3 | 1 | 1 | MPEG 2000 | ||
| 4 | 1 | 2 | MPEG 2000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 25 (mM) | ||
| 2 | 1 | reservoir | mPEG5000 | 20 (%) | |
| 3 | 1 | reservoir | MES | 50 (mM) | |
| 4 | 1 | reservoir | 200 (mM) |
