1DCT
DNA (CYTOSINE-5) METHYLASE FROM HAEIII COVALENTLY BOUND TO DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.570, 108.040, 155.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.800 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.32600 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.330 |
| Data reduction software | R-AXIS |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.076 |
| Total number of observations | 129524 * |
| Number of reflections | 26866 |
| Completeness [%] | 95.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | PEG 3500 | ||
| 3 | 1 | 1 | GLYCEROL | ||
| 4 | 1 | 1 | CACL2 | ||
| 5 | 1 | 1 | MES | ||
| 6 | 1 | 1 | DTT | ||
| 7 | 1 | 2 | WATER | ||
| 8 | 1 | 2 | PEG 3500 | ||
| 9 | 1 | 2 | GLYCEROL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MES | 100 (mM) | pH6.5 |
| 2 | 1 | reservoir | 120 (mM) | ||
| 3 | 1 | reservoir | PEG3500 | 9-13 (%) | |
| 4 | 1 | reservoir | glycerol | 13 (%) | |
| 5 | 1 | reservoir | dithiothreitol | 1 (mM) |
