1DCH

CRYSTAL STRUCTURE OF DCOH, A BIFUNCTIONAL, PROTEIN-BINDING TRANSCRIPTION COACTIVATOR

Experimental procedure

Temperature [K]	293
Collection date	1994-05-26
Spacegroup name	P 32 2 1
Unit cell lengths	105.650, 105.650, 196.230
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 3.000
R-factor	0.184
RMSD bond length	0.019
RMSD bond angle	2.900
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	3.000 *
Rmerge	0.060
Total number of observations	86670 *
Number of reflections	21436 *
Completeness [%]	82.2 *
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.2	20 *	pH 7.2 COMPND ROOM TEMP., 1.7M AMSO4, .1M HEPES, PH 7.2.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	15-20 (mg/ml)
2	1	2		5 (mM)
3	1	2	methionine	5 (mM)
4	1	2	ammonium sulfate	1.2 (M)
5	1	2	PEG400	2 (%)
6	1	2	HEPES	0.1 (M)