1DCE

CRYSTAL STRUCTURE OF RAB GERANYLGERANYLTRANSFERASE FROM RAT BRAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS II
Spacegroup name	P 1
Unit cell lengths	57.864, 77.439, 121.775
Unit cell angles	74.60, 79.91, 67.89

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.218 *
Rwork	0.215
R-free	0.26340
Structure solution method	MIR
RMSD bond length	0.016 *
RMSD bond angle	1.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.063	0.580
Total number of observations	446152 *
Number of reflections	118808 *
<I/σ(I)>	23.2	2.33
Completeness [%]	93.4	86.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5 *	21 *
1	Vapor diffusion, hanging drop *	5.5 *	21 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-10 (mg/ml)
2	1	reservoir	sodium acetate	0.1 (M)
3	1	reservoir	magnesium acetate	0.25 (M)
4	1	reservoir	sodium phosphate	10 (mM)
5	1	reservoir	ethylene glycol	6 (%)
6	1	reservoir	PEG8000	17-21 (%)