1D9E
STRUCTURE OF E. COLI KDO8P SYNTHASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-20 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 228.620, 228.620, 228.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.860 - 2.400 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.23900 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.012 * |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.860 | 2.550 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.086 | 0.564 |
| Total number of observations | 1582716 * | |
| Number of reflections | 76652 | |
| <I/σ(I)> | 22.3 | |
| Completeness [%] | 99.3 | 94.6 |
| Redundancy | 20.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 23 * | AMMONIUM SULFATE, POTASSIUM PHOSPHATE, ETHYLENE GLYCOL, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | potassium phosphate | 400 (mM) | |
| 2 | 1 | drop | ammonium sulfate | 1.4 (M) | |
| 3 | 1 | drop | ethylene glycol | 6 (%) | |
| 4 | 1 | drop | PEP | 4 (mM) | |
| 5 | 1 | drop | arabimose 5-phosphate | 4 (mM) | |
| 6 | 1 | drop | protein | 30 (mg/ml) | |
| 7 | 1 | reservoir | potassium phosphate | 400 (mM) | |
| 8 | 1 | reservoir | ethylene glycol | 6 (%) | |
| 9 | 1 | reservoir | ammonium sulfate | 1.7-1.9 (M) |
