1D6S
CRYSTAL STRUCTURE OF THE K41A MUTANT OF O-ACETYLSERINE SULFHYDRYLASE COMPLEXED IN EXTERNAL ALDIMINE LINKAGE WITH METHIONINE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-20 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-11-05 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 96.940, 149.930, 53.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.670 - 2.300 |
R-factor | 0.174 |
Rwork | 0.174 |
R-free | 0.22300 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.600 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.300 |
Rmerge | 0.105 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 297 | 30 % PEG 4000 8 % ETHANOL 100 MM TRIS (PH 7.0) 150 MM LI2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 30 (%(w/v)) | |
2 | 1 | reservoir | ethanol | 8 (%(v/v)) | |
3 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
4 | 1 | reservoir | 0.15 (M) | ||
5 | 1 | drop | protain | 20.0 (mg/ml) |