1D6A

STRUCTURE OF POKEWEED ANTIVIRAL PROTEIN COMPLEXED WITH GUANINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1999-03-29
Detector	RIGAKU RAXIS
Spacegroup name	P 1
Unit cell lengths	49.294, 49.411, 64.288
Unit cell angles	68.66, 81.04, 63.87

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.19 *
Rwork	0.190
R-free	0.25000
Starting model (for MR)	1qci
RMSD bond length	0.006
RMSD bond angle	22.000 *
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.061	0.158
Number of reflections	26047
<I/σ(I)>	21.7
Completeness [%]	87.5	80.2
Redundancy	1.71	1.61

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	16-18% PEG 4000, 0.1M CACL2, 5MM GUANINE, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15-20 (mg/ml)
2	1	reservoir	PEG4000	22 (%)
3	1	reservoir		0.1 (M)
4	1	reservoir	Tris-HCl	50 (mM)
5	1	reservoir	guanine	5 (mM)