1D5F
STRUCTURE OF E6AP: INSIGHTS INTO UBIQUITINATION PATHWAY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-28 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 101.500, 113.700, 125.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.228 |
| Rwork | 0.230 |
| R-free | 0.28300 |
| RMSD bond length | 0.012 * |
| RMSD bond angle | 1.856 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.057 | 0.420 |
| Total number of observations | 127891 * | |
| Number of reflections | 35446 | |
| <I/σ(I)> | 24 | |
| Completeness [%] | 97.7 | 99.6 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 277 | PEG1500, AMMONIUM ACETATE,SODIUM CHLORIDE, HEPES-NA, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 277 | PEG1500, AMMONIUM ACETATE,SODIUM CHLORIDE, HEPES-NA, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | drop | HEPES-Na | 50 (mM) | |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | reservoir | PEG1500 | 11-12 (%(w/v)) | |
| 6 | 1 | reservoir | ammonium acetate | 1.92 (M) | |
| 7 | 1 | reservoir | 0.3 (M) | ||
| 8 | 1 | reservoir | HEPES-Na | 0.1 (M) | |
| 9 | 1 | reservoir | dithiothreitol | 5 (mM) |
