1D4W

CRYSTAL STRUCTURE OF THE XLP PROTEIN SAP IN COMPLEX WITH SLAM PHOSPHOPEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	100.200, 49.000, 55.600
Unit cell angles	90.00, 94.20, 90.00

Refinement procedure

Resolution	10.000 - 1.800
Rwork	0.153
R-free	0.24000
RMSD bond length	0.007
RMSD bond angle	0.025
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.800
Rmerge	0.076
Total number of observations	113987 *
Number of reflections	23946 *
Completeness [%]	94.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	295	30% PEG 8000, 20% GLYCEROL, 100 MM SODIUM CITRATE, 10MM DTT, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 22K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	HEPES	20 (mM)
3	1	drop		150 (mM)
4	1	drop	dithiothreitol	10 (mM)
5	1	reservoir	PEG8000	30 (%)
6	1	reservoir	glycerol	20 (%)
7	1	reservoir	sodium citrate	100 (mM)
8	1	reservoir	dithiothreitol	10 (mM)