1D4V
Crystal structure of trail-DR5 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 95.520, 95.520, 69.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.200 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.27000 |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | |
| High resolution limit [Å] | 2.200 | |
| Rmerge | 0.082 | 0.760 * |
| Total number of observations | 164565 * | |
| Number of reflections | 18104 | |
| <I/σ(I)> | 30 | |
| Completeness [%] | 96.0 | 80 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 25% ETHYLENE GLYCOL, 0.1% N-OCTYL-BETA-GLUCOSIDE, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 20K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | reservoir | ethylene glycol | 25 (%) | |
| 5 | 1 | reservoir | n-octyl-beta-glucoside | 0.1 (%) |
