1D3B
CRYSTAL STRUCTURE OF THE D3B SUBCOMPLEX OF THE HUMAN CORE SNRNP DOMAIN AT 2.0A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 107.350, 108.450, 110.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| Rwork | 0.213 |
| R-free | 0.26500 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.034 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.200 | 2.200 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.093 * | 0.450 * |
| Number of reflections | 89816 | |
| <I/σ(I)> | 6.1 | 1.5 |
| Completeness [%] | 99.2 | 98.8 |
| Redundancy | 4.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8.5 | pH 8.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Na/K phosphate | 1.8 (M) | |
| 2 | 1 | reservoir | glycerol | 15 (%) | |
| 3 | 1 | reservoir | spermidine | 10 (mM) |
