1D2H
CRYSTAL STRUCTURE OF R175K MUTANT GLYCINE N-METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLHOMOCYSTEINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-07-10 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 43 |
| Unit cell lengths | 77.900, 77.900, 227.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.000 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.24700 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.230 |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 3.200 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.077 | 0.190 |
| Total number of observations | 99483 * | |
| Number of reflections | 25574 | |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 92.5 | 91 |
| Redundancy | 3.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | PEG-4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | AdoHcy | 5 (mM) | |
| 2 | 1 | drop | sodium citrate | 100 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | PEG3400 | 10 (%) | |
| 5 | 1 | drop | protain | 30 (mg/ml) |
