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1D1J

CRYSTAL STRUCTURE OF HUMAN PROFILIN II

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X9B
Synchrotron siteNSLS
BeamlineX9B
Temperature [K]100
Detector technologyCCD
Collection date1999-04-21
DetectorADSC QUANTUM 4
Spacegroup nameP 1 21 1
Unit cell lengths71.140, 44.800, 89.600
Unit cell angles90.00, 98.59, 90.00
Refinement procedure
Resolution20.000 - 2.200
R-factor0.22
Rwork0.216
R-free0.26780

*

RMSD bond length0.007
RMSD bond angle1.340
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareX-PLOR (3.851)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.000

*

2.280
High resolution limit [Å]2.2002.200
Rmerge0.0890.185
Total number of observations430124

*

Number of reflections28050
<I/σ(I)>10.3
Completeness [%]96.9

*

85.3

*

Redundancy2.72.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.34

*

MAGNESIUM SULFATE, PEG400, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11reservoir1.35 (M)
21reservoirPEG4001.5 (%(v/v))
31reservoirHEPES100 (mM)

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