1D1J
CRYSTAL STRUCTURE OF HUMAN PROFILIN II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-04-21 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.140, 44.800, 89.600 |
Unit cell angles | 90.00, 98.59, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.22 |
Rwork | 0.216 |
R-free | 0.26780 * |
RMSD bond length | 0.007 |
RMSD bond angle | 1.340 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 * | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.089 | 0.185 |
Total number of observations | 430124 * | |
Number of reflections | 28050 | |
<I/σ(I)> | 10.3 | |
Completeness [%] | 96.9 * | 85.3 * |
Redundancy | 2.7 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 4 * | MAGNESIUM SULFATE, PEG400, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 1.35 (M) | ||
2 | 1 | reservoir | PEG400 | 1.5 (%(v/v)) | |
3 | 1 | reservoir | HEPES | 100 (mM) |