1CZ8
VASCULAR ENDOTHELIAL GROWTH FACTOR IN COMPLEX WITH AN AFFINITY MATURED ANTIBODY
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-01-28 |
Detector | ADSC |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 89.120, 66.400, 138.750 |
Unit cell angles | 90.00, 94.70, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.400 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.26700 |
RMSD bond length | 0.012 |
RMSD bond angle | 29.600 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | X-PLOR |
Refinement software | X-PLOR (98.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.076 | 0.380 |
Total number of observations | 209257 * | |
Number of reflections | 61742 | 8900 * |
<I/σ(I)> | 13.6 | |
Completeness [%] | 97.4 | 96.7 |
Redundancy | 3.4 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 6 | 293 | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7.8 (A280) | |
2 | 1 | drop | Tris-HCl | 30 (mM) | |
3 | 1 | drop | sodium chloride | 0.4 (M) | |
4 | 1 | reservoir | PEG | 15 (%) | |
5 | 1 | reservoir | isopropanol | 10 (%) | |
6 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
7 | 1 | reservoir | MES | 0.2 (M) |