1CZ3

DIHYDROFOLATE REDUCTASE FROM THERMOTOGA MARITIMA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	291
Detector technology	IMAGE PLATE
Collection date	1997-06-07
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	131.605, 36.051, 97.025
Unit cell angles	90.00, 130.69, 90.00

Refinement procedure

Resolution	9.010 - 2.100
R-factor	0.203
Rwork	0.203
R-free	0.25500
RMSD bond length	0.010
RMSD bond angle	1.600
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9.010 *	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.132	0.517
Total number of observations	60564 *
Number of reflections	19395
<I/σ(I)>	8.91
Completeness [%]	93.9	87.3
Redundancy	3.2	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7	18 *	2 M (NH4)2SO4 25 MM TRIS/HCL 0.5 MM EDTA, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.5 (mg/ml)
2	1	drop	ammonium sulfate	2 (M)
3	1	drop	Tris-HCl	25 (mM)
4	1	drop	EDTA	0.5 (mM)