1CZ3
DIHYDROFOLATE REDUCTASE FROM THERMOTOGA MARITIMA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 291 |
Detector technology | IMAGE PLATE |
Collection date | 1997-06-07 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 131.605, 36.051, 97.025 |
Unit cell angles | 90.00, 130.69, 90.00 |
Refinement procedure
Resolution | 9.010 - 2.100 |
R-factor | 0.203 |
Rwork | 0.203 |
R-free | 0.25500 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.600 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 9.010 * | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.132 | 0.517 |
Total number of observations | 60564 * | |
Number of reflections | 19395 | |
<I/σ(I)> | 8.91 | |
Completeness [%] | 93.9 | 87.3 |
Redundancy | 3.2 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7 | 18 * | 2 M (NH4)2SO4 25 MM TRIS/HCL 0.5 MM EDTA, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.5 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 2 (M) | |
3 | 1 | drop | Tris-HCl | 25 (mM) | |
4 | 1 | drop | EDTA | 0.5 (mM) |