1CXV

STRUCTURE OF RECOMBINANT MOUSE COLLAGENASE-3 (MMP-13)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 1 21 1
Unit cell lengths	56.360, 48.460, 74.940
Unit cell angles	90.00, 108.15, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.196
Rwork	0.196
R-free	0.23400
RMSD bond length	0.009
RMSD bond angle	2.100
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	2.000 *	2.000 *
Rmerge	0.075 *	0.286 *
Total number of observations	165387 *
Number of reflections	25433 *
Completeness [%]	96.8 *	94.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7.5 *	18 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	17 (mg/ml)
2	1	1	HEPES	10 (mM)
3	1	1	ammonium sulfate		saturated