1CWS
HUMAN CDC25B CATALYTIC DOMAIN WITH TUNGSTATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | SIEMENS |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Collection date | 1997-04-28 |
Detector | SIEMENS HI-STAR |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.734, 71.863, 74.935 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.000 |
R-factor | 0.142 * |
R-free | 0.18700 * |
Structure solution method | MIR |
RMSD bond length | 0.015 * |
RMSD bond angle | 2.300 * |
Data reduction software | SADIE |
Data scaling software | SAINT |
Phasing software | XTAL (V. 3.0) |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.081 |
High resolution limit [Å] | 2.000 | 2.008 |
Rmerge | 0.071 * | 0.280 * |
Total number of observations | 80685 * | |
Number of reflections | 16621 | |
<I/σ(I)> | 18.4 | 2 |
Completeness [%] | 86.0 | 47.1 * |
Redundancy | 4.86 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 9 | 4.0 * | 4 MICROLITERS PROTEIN (10 MG/ ML IN 50 MM TRISHCL, WO4=,1% BME AT PH 9.0) MIXED WITH 4 MICROLITERS WELL BUFFER (1.8 (NH4)2SO4, 0.5 M NACL, 0.1 M TRISHCL, 0.25 BME) AT 4 DEG. C |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | |
3 | 1 | drop | beta-mercaptoethanol | 1 (%(v/v)) | |
4 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
5 | 1 | reservoir | 0.5 (M) | ||
6 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
7 | 1 | reservoir | BME | 0.25 (%) |