1CVX
CRYSTAL STRUCTURE OF POLYAMIDE DIMER (IMPYHPPYBETADP)2 BOUND TO B-DNA DECAMER CCAGATCTGG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 31 |
| Unit cell lengths | 34.200, 34.200, 46.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.000 - 2.270 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.23800 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.000 | 2.350 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.023 | 0.204 |
| Total number of observations | 7261 * | |
| Number of reflections | 2680 | |
| <I/σ(I)> | 42.3 | |
| Completeness [%] | 96.1 | 87 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 4 * | CALCIUM ACETATE, TRIS, MPD, pH 8.1, VAPOR DIFFUSION, SITTING DROP, temperature 277.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | CALCIUM ACETATE | ||
| 2 | 1 | 1 | TRIS | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | duplex DNA | 0.6 (mM) | |
| 2 | 1 | drop | polyamide | 1.3 (mM) | |
| 3 | 1 | drop | 12 (%) | ||
| 4 | 1 | drop | Ca acetate | 35 (mM) | |
| 5 | 1 | drop | Tris-HCl | 10 (mM) | |
| 6 | 1 | reservoir | MPD | 25 (%) |
