1CVS
CRYSTAL STRUCTURE OF A DIMERIC FGF2-FGFR1 COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | AREA DETECTOR |
| Collection date | 1999-04-25 |
| Detector | SDMS |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 98.450, 98.450, 197.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.800 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMEN |
| Starting model (for MR) | FGF2 TELOKIN |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.930 * |
| Data reduction software | SDMS |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.072 | 0.255 |
| Total number of observations | 196126 * | |
| Number of reflections | 24726 * | |
| <I/σ(I)> | 18.3 | |
| Completeness [%] | 99.8 | 98.1 |
| Redundancy | 7.9 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 20 * | ammonium sulfate, glycerol, Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 25 (mM) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
| 5 | 1 | reservoir | glycerol | 20 (%) | |
| 6 | 1 | reservoir | Tris-HCl | 0.1 (M) |
