1CUL

COMPLEX OF GS-ALPHA WITH THE CATALYTIC DOMAINS OF MAMMALIAN ADENYLYL CYCLASE: COMPLEX WITH 2',5'-DIDEOXY-ADENOSINE 3'-TRIPHOSPHATE AND MG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	1998-06-04
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 2
Unit cell lengths	118.200, 133.600, 70.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.400
R-factor	0.221
Rwork	0.216
R-free	0.26300
RMSD bond length	0.007
RMSD bond angle	1.220
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.117	0.206
Number of reflections	36169
<I/σ(I)>	5.4
Completeness [%]	82.4	35.9
Redundancy	2.7	1.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	PROTEIN MIXED 1:1 WITH WELL SOLUTION *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	PEG8000	7.2-7.5 (%)
3	1	reservoir		500 (mM)
4	1	reservoir	MES	100 (mM)