1CT8
CATALYTIC ANTIBODY 7C8 COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 278 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.558, 65.612, 117.761 |
| Unit cell angles | 90.00, 95.56, 90.00 |
Refinement procedure
| Resolution | 14.300 - 2.200 |
| R-factor | 0.211 * |
| Rwork | 0.211 |
| R-free | 0.27500 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.750 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.300 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.066 | 0.253 |
| Total number of observations | 98277 * | |
| Number of reflections | 40238 | |
| <I/σ(I)> | 15.2 | |
| Completeness [%] | 97.9 | 95.7 |
| Redundancy | 2.44 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 4 | 291 | used macroseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | Fab | 5 (mg/ml) | |
| 2 | 1 | 1 | 150 (mM) | ||
| 3 | 1 | 2 | PEG1000 | 22-26 (%(w/v)) | |
| 4 | 1 | 2 | ammonium sulfate | 0.2 (M) |
