1CT8

CATALYTIC ANTIBODY 7C8 COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	278
Detector technology	IMAGE PLATE
Collection date	1997-04-08
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	54.558, 65.612, 117.761
Unit cell angles	90.00, 95.56, 90.00

Refinement procedure

Resolution	14.300 - 2.200
R-factor	0.211 *
Rwork	0.211
R-free	0.27500
RMSD bond length	0.011
RMSD bond angle	1.750
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	14.300	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.066	0.253
Total number of observations	98277 *
Number of reflections	40238
<I/σ(I)>	15.2
Completeness [%]	97.9	95.7
Redundancy	2.44	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4	291	used macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Fab	5 (mg/ml)
2	1	1		150 (mM)
3	1	2	PEG1000	22-26 (%(w/v))
4	1	2	ammonium sulfate	0.2 (M)