1CSB
Crystal structure of cathepsin b inhibited with CA030 at 2.1 angstroms resolution: A basis for the design of specific epoxysuccinyl inhibitors
Experimental procedure
Detector technology | IMAGE PLATE |
Collection date | 1993-11 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 86.510, 34.150, 85.530 |
Unit cell angles | 90.00, 103.10, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.000 |
R-factor | 0.194 |
Rwork | 0.194 |
RMSD bond length | 0.011 * |
RMSD bond angle | 1.580 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 2.000 * | 2.000 * |
Rmerge | 0.098 | |
Number of reflections | 51421 | |
Completeness [%] | 79.1 | 64 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5 * | 21 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 1.5 (M) | |
2 | 1 | reservoir | sodium acetate | 0.1 (M) | |
3 | 1 | drop | protein | 5 (mg/ml) |