1CR2
CRYSTAL STRUCTURE OF THE HELICASE DOMAIN OF THE GENE 4 PROTEIN OF BACTERIOPHAGE T7: COMPLEX WITH DATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 1999-03-03 |
| Detector | BRANDEIS - B4 |
| Spacegroup name | P 61 |
| Unit cell lengths | 80.172, 80.172, 85.709 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.25 |
| Rwork | 0.245 |
| R-free | 0.29600 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.460 |
| High resolution limit [Å] | 2.300 | 2.310 |
| Rmerge | 0.063 | 0.149 |
| Total number of observations | 153764 * | |
| Number of reflections | 13072 | |
| <I/σ(I)> | 28 | |
| Completeness [%] | 96.1 | 100 |
| Redundancy | 11.7 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 298 | ammonium sulfate, ACES, pH 9.00, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25-35 (mg/ml) | |
| 2 | 1 | drop | lithium sulfate | 10 (mM) | |
| 3 | 1 | drop | Tris-HCl | 20 (mM) | |
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.4-1.6 (M) | |
| 6 | 1 | reservoir | ACES | 100 (mM) |
