1CR2
CRYSTAL STRUCTURE OF THE HELICASE DOMAIN OF THE GENE 4 PROTEIN OF BACTERIOPHAGE T7: COMPLEX WITH DATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 1999-03-03 |
Detector | BRANDEIS - B4 |
Spacegroup name | P 61 |
Unit cell lengths | 80.172, 80.172, 85.709 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.25 |
Rwork | 0.245 |
R-free | 0.29600 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.900 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.460 |
High resolution limit [Å] | 2.300 | 2.310 |
Rmerge | 0.063 | 0.149 |
Total number of observations | 153764 * | |
Number of reflections | 13072 | |
<I/σ(I)> | 28 | |
Completeness [%] | 96.1 | 100 |
Redundancy | 11.7 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 298 | ammonium sulfate, ACES, pH 9.00, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 25-35 (mg/ml) | |
2 | 1 | drop | lithium sulfate | 10 (mM) | |
3 | 1 | drop | Tris-HCl | 20 (mM) | |
4 | 1 | drop | dithiothreitol | 5 (mM) | |
5 | 1 | reservoir | ammonium sulfate | 1.4-1.6 (M) | |
6 | 1 | reservoir | ACES | 100 (mM) |