1CQT
CRYSTAL STRUCTURE OF A TERNARY COMPLEX CONTAINING AN OCA-B PEPTIDE, THE OCT-1 POU DOMAIN, AND AN OCTAMER ELEMENT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-09-18 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 93.722, 93.722, 152.651 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.200 |
| R-factor | 0.264 |
| Rwork | 0.264 |
| R-free | 0.32600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.350 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.084 | 0.375 |
| Total number of observations | 175486 * | |
| Number of reflections | 11803 | |
| <I/σ(I)> | 22.45 | |
| Completeness [%] | 99.5 | 97.8 |
| Redundancy | 14.8 | 5.89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 293 | pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | peptide | 1.0 (mM) | |
| 2 | 1 | drop | domain | 1.0 (mM) | |
| 3 | 1 | drop | oligonucleotide | 1.2 (mM) | |
| 4 | 1 | drop | 33 (mM) | ||
| 5 | 1 | drop | HEPES | 50 (mM) | |
| 6 | 1 | drop | 10 (mM) | ||
| 7 | 1 | drop | PEG3350 | 24-26 (%) |
