1COY

CRYSTAL STRUCTURE OF CHOLESTEROL OXIDASE COMPLEXED WITH A STEROID SUBSTRATE. IMPLICATIONS FOR FAD DEPENDENT ALCOHOL OXIDASES

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	67.930, 84.230, 87.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.159
Rwork	0.159
RMSD bond length	0.010
RMSD bond angle	1.350
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.066 *
Total number of observations	192439 *
Number of reflections	46169 *
Completeness [%]	96.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		18 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	sodium/potassium phosphate	0.5-0.65 (M)
3	1	reservoir	sodium/potassium phosphate	1.3 (M)