1CNT
CILIARY NEUROTROPHIC FACTOR
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Detector technology | IMAGE PLATE |
| Collection date | 1994 |
| Wavelength(s) | 1.37, 1.39 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.950, 62.090, 208.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.24700 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.087 |
| Data reduction software | DENZO |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.055 |
| Total number of observations | 40431 * |
| Number of reflections | 18227 * |
| Completeness [%] | 92.8 * |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.35 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 3.75 (%) | |
| 3 | 1 | drop | ammonium sulfate | 0.075 (M) | |
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | sodium citrate | 25 (mM) | |
| 6 | 1 | reservoir | PEG8000 | 7.5 (%) | |
| 7 | 1 | reservoir | ammonium sulfate | 0.15 (M) | |
| 8 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 9 | 1 | reservoir | sodium citrate | 50 (mM) |
