1CM8
PHOSPHORYLATED MAP KINASE P38-GAMMA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.500, 66.820, 206.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.232 * |
Rwork | 0.232 |
R-free | 0.28260 |
Structure solution method | MIR |
RMSD bond length | 0.010 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS (0.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 2.400 | 2.400 * |
Rmerge | 0.067 * | |
Total number of observations | 118429 * | |
Number of reflections | 31732 | |
<I/σ(I)> | 9.8 | |
Completeness [%] | 89.9 | 76.5 * |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 * | pH 7.0, VAPOR DIFFUSION |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.5 (mM) | |
2 | 1 | drop | AMP-PNP | 5 (mM) | |
3 | 1 | drop | 0.02 (%) | ||
4 | 1 | reservoir | 100 (mM) | ||
5 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
6 | 1 | reservoir | PEG4000 | 27 (%) | |
7 | 1 | reservoir | 10 (mM) | ||
8 | 1 | reservoir | dithiothreitol | 5 (mM) |