1CKN
STRUCTURE OF GUANYLYLATED MRNA CAPPING ENZYME COMPLEXED WITH GTP
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-01 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 94.906, 212.953, 105.016 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.500 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.29900 |
Starting model (for MR) | 1ckm |
RMSD bond length | 0.012 |
RMSD bond angle | 25.170 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 15.000 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.052 |
Number of reflections | 36109 |
<I/σ(I)> | 13.1 |
Completeness [%] | 97.6 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 7.5 | Doherty, A.J., (1996) Nucl. Acids Res., 24. 2281. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | clostripain | 2.5 (U/ml) | |
2 | 1 | 1 | Tris-HCl | 50 (mM) | |
3 | 1 | 1 | 5 (mM) | ||
4 | 1 | 1 | dithiothreitol | 5 (mM) | |
5 | 1 | 1 | glycerol | 10 (%) |