1CKM
STRUCTURE OF TWO DIFFERENT CONFORMATIONS OF MRNA CAPPING ENZYME IN COMPLEX WITH GTP
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-07 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 93.332, 214.931, 105.752 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.29900 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT WITH A MERCURY DERIVATIVE OBTAINED BY SOAKING THE CRYSTAL FOR TWO HOURS IN 1 MM THIMEROSAL AND A SELENOMETHIONINE DERIVATIVE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 25.164 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.062 |
| Number of reflections | 36196 |
| <I/σ(I)> | 13.3 |
| Completeness [%] | 97.5 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 | Doherty, A.J., (1996) Nucl. Acids Res., 24. 2281. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | clostripain | 2.5 (U/ml) | |
| 2 | 1 | 1 | Tris-HCl | 50 (mM) | |
| 3 | 1 | 1 | 5 (mM) | ||
| 4 | 1 | 1 | dithiothreitol | 5 (mM) | |
| 5 | 1 | 1 | glycerol | 10 (%) |
