1CKG

T52V MUTANT HUMAN LYSOZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	283
Detector technology	IMAGE PLATE
Collection date	1998-02-01
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 21
Unit cell lengths	65.257, 106.667, 39.542
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.200
Rwork	0.166
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	WILD-TYPE OF HUMAN LYSOZYME
RMSD bond length	0.009
RMSD bond angle	24.900 *
Data reduction software	PROCESS
Data scaling software	PROCESS
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.090
High resolution limit [Å]	2.000	2.000
Rmerge	0.100	0.239
Total number of observations	31949 *
Number of reflections	16216
<I/σ(I)>		3.3
Completeness [%]	83.6	61.1
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5 *		protein solution is mixed in a 1:1 ratio with well solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir		2.5 (M)
3	1	reservoir	acetate	20 (mM)