1CHD

CHEB METHYLESTERASE DOMAIN

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1994-08-16
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 32 2 1
Unit cell lengths	63.380, 63.380, 87.010
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 1.750
R-factor	0.182
R-free	0.23000
RMSD bond length	0.009 *
RMSD bond angle	0.030 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.750 *
Rmerge	0.045	0.154 *
Number of reflections	19729
Completeness [%]	93.6	89.3 *
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.0-1.2 (M)
2	1	reservoir	Tris-HCl	0.1 (M)
3	1	reservoir	glycerol	12 (%(v/v))
4	1	reservoir	2-mercaptoethanol	1.4 (mM)
5	1	reservoir		5 (mM)