1CFY
YEAST COFILIN, MONOCLINIC CRYSTAL FORM
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 290 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995 |
| Detector | SIEMENS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.089, 30.767, 105.940 |
| Unit cell angles | 90.00, 133.15, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| Rwork | 0.175 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cof |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.024 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | X-PLOR |
| Refinement software | PROFFT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 21.600 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.051 |
| Number of reflections | 13627 |
| <I/σ(I)> | 19.8 |
| Completeness [%] | 82.8 |
| Redundancy | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 | CRYSTALLIZED FROM 30% PEG 4000 SOLUTION., pH 7.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 20-30 (%) |
