1CFY

YEAST COFILIN, MONOCLINIC CRYSTAL FORM

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	290
Detector technology	AREA DETECTOR
Collection date	1995
Detector	SIEMENS
Spacegroup name	C 1 2 1
Unit cell lengths	152.089, 30.767, 105.940
Unit cell angles	90.00, 133.15, 90.00

Refinement procedure

Resolution	8.000 - 2.300
Rwork	0.175
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cof
RMSD bond length	0.008
RMSD bond angle	0.024
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	X-PLOR
Refinement software	PROFFT

Data quality characteristics

	Overall
Low resolution limit [Å]	21.600
High resolution limit [Å]	2.300
Rmerge	0.051
Number of reflections	13627
<I/σ(I)>	19.8
Completeness [%]	82.8
Redundancy	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2		CRYSTALLIZED FROM 30% PEG 4000 SOLUTION., pH 7.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	20-30 (%)