1CF3
GLUCOSE OXIDASE FROM APERGILLUS NIGER
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 290 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 67.540, 67.540, 215.380 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.24300 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gal |
RMSD bond length | 0.008 |
RMSD bond angle | 0.023 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.990 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.054 | 0.122 |
Number of reflections | 40401 | |
<I/σ(I)> | 21.5 | 6.9 |
Completeness [%] | 87.7 | 78.7 |
Redundancy | 2.5 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.6 | Kalisz, H.M., (1990) J.Mol.Biol., 213, 207. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | glucose oxidase | 20 (mg/ml) | |
2 | 1 | drop | acetate | 50 (mM) | |
3 | 1 | drop | ammonium sulfate | 1.6 (M) | |
4 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
5 | 1 | drop | octanetriol solution | saturated |