1CEH
STRUCTURE AND FUNCTION OF THE CATALYTIC SITE MUTANT ASP99ASN OF PHOSPHOLIPASE A2: ABSENCE OF CONSERVED STRUCTURAL WATER
Experimental procedure
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 46.110, 46.110, 102.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.500 - 1.900 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.900 * |
| Rmerge | 0.060 * |
| Number of reflections | 10307 |
| Completeness [%] | 98.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.6 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | Tris | 100 (mM) | |
| 2 | 1 | 1 | 5 (mM) | ||
| 3 | 1 | 1 | protein | ||
| 4 | 1 | 2 | MPD | 30 (mg/ml) | 0.05ml |
