1CE1

1.9A STRUCTURE OF THE THERAPEUTIC ANTIBODY CAMPATH-1H FAB IN COMPLEX WITH A SYNTHETIC PEPTIDE ANTIGEN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	100
Collection date	1998-02
Spacegroup name	P 21 21 21
Unit cell lengths	54.846, 60.672, 152.908
Unit cell angles	90.00, 89.72, 89.85

Refinement procedure

Resolution	8.000 * - 1.900
R-factor	0.208 *
Rwork	0.220
R-free	0.26500 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.600 *
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.062 *	0.269 *
Number of reflections	40254
Completeness [%]	98.8	95.1
Redundancy	3	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5	23 *	pH 8.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	heptapeptide	10 (mM)
2	1	reservoir	PEG10000	20 (%)