1CDO
ALCOHOL DEHYDROGENASE (E.C.1.1.1.1) (EE ISOZYME) COMPLEXED WITH NICOTINAMIDE ADENINE DINUCLEOTIDE (NAD), AND ZINC
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 102.950, 47.600, 80.430 |
| Unit cell angles | 90.00, 104.66, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.050 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.24800 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 25.100 * |
| Data reduction software | DENZO |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 2.050 * | 2.050 * |
| Rmerge | 0.120 | |
| Total number of observations | 83875 * | |
| Number of reflections | 39749 | |
| Completeness [%] | 86.9 | 73.7 * |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.9 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | TES | 50 (mM) | |
| 2 | 1 | reservoir | NAD | 10 (mM) | |
| 3 | 1 | reservoir | pyrazole | 1 (mM) | |
| 4 | 1 | reservoir | PEG2000 | 15.2 (%) |
