1CC0

CRYSTAL STRUCTURE OF THE RHOA.GDP-RHOGDI COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-11-15
Detector	MARRESEARCH
Spacegroup name	P 65 2 2
Unit cell lengths	139.300, 139.300, 253.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	? - 5.000
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	4.090
High resolution limit [Å]	4.000	4.000
Rmerge	0.061 *	0.193 *
Total number of observations	130318 *
Number of reflections	12817
<I/σ(I)>	12.1
Completeness [%]	99.1	99.6
Redundancy	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.2 *	293	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	Tris-HCl	25 (mM)
3	1	drop		100 (mM)
4	1	drop		5 (mM)
5	1	reservoir	ammonium sulfate	51 (%sat)
6	1	reservoir		100 (mM)