1CBU
ADENOSYLCOBINAMIDE KINASE/ADENOSYLCOBINAMIDE PHOSPHATE GUANYLYLTRANSFERASE (COBU) FROM SALMONELLA TYPHIMURIUM
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 263 |
Detector technology | AREA DETECTOR |
Collection date | 1997-09 |
Detector | SIEMENS HI-STAR |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 96.400, 114.400, 106.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 18.500 * |
Data reduction software | XDS |
Data scaling software | XCALIBRE |
Phasing software | HEAVY |
Refinement software | TNT (4C) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 2.370 | |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.043 * | 0.210 * |
Number of reflections | 24136 | |
<I/σ(I)> | 16 | 1.7 |
Completeness [%] | 88.5 | 64.4 |
Redundancy | 3 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 5.5 | 4 * | PROTEIN WAS CRYSTALLIZED BY MICRO BATCH FROM 6% PEG 3350, 200 MM NACL AND 50 MM SUCCINATE, PH 5.5 AT 4 C., micro batch |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | PEG3350 | 6 (%) | |
2 | 1 | 1 | 200 (mM) | ||
3 | 1 | 1 | succinate | 50 (mM) | |
4 | 1 | 1 | protein | 5-8 (mg/ml) |